Factors Associated with Treatment Outcome of Carbon Dioxide Laser for Trigeminal Neuralgia

Abstract:Objective: To report the treatment outcome and identify factors associated with treatmentoutcome of using carbon dioxide (CO2) laser neural ablation for trigeminal neuralgia (TN).Materials and methods: This was a retrospective study of 36 patients who underwent CO2laser neural ablation to treat TN. The medical records were reviewed and analyzed. Pain relief for atleast 1 year after the procedure would be classified as treatment success.Results: For the total of 36 patients, 15 of them (41.7%) achieved the treatment success.Regarding the factors associated with the treatment outcome, the total daily dose of carbamazepineof 600 mg or less per day and shorter duration of symptoms prior to receiving the operation(persistent symptom of 3 years or less) were related to success of treatment. The odds ratios ofboth factors were 12 (95% CI = 1.6-87.9) for the carbamazepine dose, and 16 (95% CI = 2.2-112.9)for the duration of persistent symptom of 3 years or less.Conclusions: The CO2 laser neural ablation decreased the pain symptoms of the patientswith TN. The preoperative dose of carbamazepine and the duration of symptoms prior to treatmentwere associated with good treatment outcomes of CO2 laser for TN.Keywords: Trigeminal neuralgia, Tic Douloureux, Carbone dioxide laser, LasersSWU Dent J. 2022;15(2):79-88

Tooth Transplantation Using Computer-Aided Rapid Prototyping Model Compared to Conventional Technique (A Pilot Study)

Objective: This research study aimed to compare the efficiency between tooth transplantation using the Computer-aided rapid prototyping model (CARP model) and a conventional tooth transplantation technique. Materials & Methods: Ten patients were enrolled in this study. Patients were randomly divided into 2 groups. Five patients were performed tooth transplantation using the CARP technique (study group) and other five patients were performed antotransplantation using the conventional technique (controlled group). During transplantation, operation time, extra-alveolar time, and attempt of fitting donor tooth to recipient site were evaluated. Moreover, after 3 months post-operation, PDL space, tooth mobility, and pocket depth were examined. Result: During transplantation, the study group consumed lower operating time and extraalveolar time compared to the control group although no statistic significance was found (p = 0.086 and p = 0.05 respectively). In addition, the study group showed significantly fewer attempts to fit the donor tooth to the recipient socket compared to the control group (p = 0.019). After 3 months post-transplantation, average PDL width shows a narrower significant difference in the study group compared to the control group (p = 0.014). Moreover, the study group showed significantly better pocket depth reduction compared to the control group (p = 0.024). No significant difference found in tooth mobility after tooth transplantation in both groups (p = 0.074). Conclusion: CARP technique reduced attempt to fitting donor tooth and improved PDL healing of donor tooth in tooth transplantation compared to conventional technique

6-Bromoindirubin-3′-Oxime Regulates Colony Formation, Apoptosis, and Odonto/Osteogenic Differentiation in Human Dental Pulp Stem Cells

International audience6-bromoindirubin-3′-oxime (BIO) is a candidate small molecule that effectively modulates Wnt signalling owing to its stable property. The present study investigated the influence of BIO on the odonto/osteogenic differentiation of human dental pulp stem cells (hDPSCs). hDPSCs were treated with 200, 400, or 800 nM BIO, and the effects on hDPSC responses and osteogenic differentiation were assessed. BIO-mediated Wnt activation was confirmed by β-catenin nuclear translocation detected by immunofluorescence staining. BIO attenuated colony formation and cell migration determined by in vitro wound-healing assay. BIO increased early apoptotic cell population evaluated using flow cytometry. For osteogenic induction, BIO promoted alkaline phosphatase (ALP) activity and mineralisation in a dose-dependent manner. ALP, RUNX2, OCN, OSX, ANKH, DMP1, and DSPP mRNA expression were significantly upregulated. The OPG/RANKL expression ratio was also increased. Further, BIO attenuated adipogenic differentiation as demonstrated by decreased lipid accumulation and adipogenic-related gene expression. Bioinformatic analysis of RNA sequencing data from the BIO-treated hDPSCs revealed that BIO modulated pathways related to autophagy and actin cytoskeleton regulation. These findings demonstrated that BIO treatment promoted hDPSC osteogenic differentiation. Therefore, this small molecule is a strong candidate as a bioactive molecule to enhance dentin repai